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H7665

Sigma-Aldrich

Anti-Histone H1.4 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-H1 Histone family, member 4, Anti-H1.4, Anti-H1F4, Anti-HIST1H1E, Anti-Histone H1b, Anti-Histone I family, 4, Anti-Histone cluster 1 H1e

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~35 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

western blot: 1-2 μg/mL using acid-extracted fraction of HL60 cells

UniProt accession no.

P10412

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HIST1H1E(3008)

General description

In mammalian cells, four histone H1 variants (H1.2 to H1.5) are found in all somatic cells, and a fifth (H1.1) is present in thymus, testis and spleen, lymphocytic and neuronal cells. K26/H1.4 is present within the flexible N-terminal domain of H1.4 just before the globular domain. Histone modifications are thought to play an important role in cancer and disease.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Linker histone H1 binds to DNA between nucleosomal core particles and is involved in establishing and maintaining higher order chromatin structures. It is covalently modified. Histone H1 phosphorylation occurs at multiple sites including at Ser27 residue. Histone H1.4 is di-methylated or acetylated at Lys26. enhancer of Zeste 2 (Ezh2).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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HP1 binds specifically to Lys26-methylated histone H1. 4, whereas simultaneous Ser27 phosphorylation blocks HP1 binding
Daujat S, et al.
Test, 280(45), 38090-38095 (2005)
Lucy A McNamara et al.
Journal of virology, 86(17), 9337-9350 (2012-06-22)
The ability of HIV-1 to establish a latent infection presents a barrier to curing HIV. The best-studied reservoir of latent virus in vivo is resting memory CD4(+) T cells, but it has recently been shown that CD34(+) hematopoietic progenitor cells
Sarka Jelinkova et al.
International journal of molecular sciences, 22(9) (2021-06-03)
Duchenne muscular dystrophy (DMD) is a devastating condition shortening the lifespan of young men. DMD patients suffer from age-related dilated cardiomyopathy (DCM) that leads to heart failure. Several molecular mechanisms leading to cardiomyocyte death in DMD have been described. However
Sullivan Renouard et al.
BMC research notes, 5, 15-15 (2012-01-11)
While seed biology is well characterized and numerous studies have focused on this subject over the past years, the regulation of seed coat development and metabolism is for the most part still non-elucidated. It is well known that the seed
Jikui Xiong et al.
Oncology letters, 15(4), 4767-4774 (2018-03-20)
Malignant melanoma is characterized by rapid deterioration, early metastasis and high mortality. Cdk5 regulatory subunit-associated protein 1 (CDK5RAP1), which catalyzes 2-methylthio (ms2) modification of mitochondrial transfer RNAs, has been reported to induce cancer cell apoptosis, by a phospho-c-Jun N-terminal kinase
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