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GE17-0430-01

CNBr Activated Sepharose™ 4B

Cytiva 17-0430-01, pack of 15 g

Synonym(s):

CNBr Sepharose 4B

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

packaging

pack of 15 g

manufacturer/tradename

Cytiva 17-0430-01

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

ligand dependent

2-11

storage temp.

2-8°C

General description

CNBr-activated Sepharose™ 4B is a pre-activated media used for coupling antibodies or other large proteins containing -NH? groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.

CNBr-activated Sepharose™ 4B is a pre-activated medium for immobilization of ligands containing primary amines. It provides a very convenient way to immobilize ligands by the cyanogen bromide method. The coupling reaction is spontaneous, rapid and easy to carry out. The application area covers immobilization of proteins, peptides and nucleic acids.

Features and Benefits

  • For immobilizing antibodies or other large proteins containing -NH2 groups by coupling them to the matrix
  • Over 25 years proven track record for coupling ligands containing primary amino groups
  • Multipoint attachment resulting in a chemically stable product
  • The coupling reaction by the cyanogen bromide method is spontaneous, rapid and easy to carry out

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Arina L Maltseva et al.
Frontiers in physiology, 5, 497-497 (2015-01-08)
Immune responses of invertebrate animals are mediated through innate mechanisms, among which production of antimicrobial peptides play an important role. Although evolutionary Polychaetes represent an interesting group closely related to a putative common ancestor of other coelomates, their immune mechanisms

Protocols

Coupling through the Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service