G6637

β-Galactose Dehydrogenase from Pseudomonas fluorescens

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein (biuret)

• Synonym(s): D-Galactose:NAD⁺ 1-oxidoreductase
• CAS Number: 9028-54-0
• Enzyme Commission number: 1.1.1.48 (BRENDA, IUBMB)
• EC Number: 232-837-4
• MDL number: MFCD00130622
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Pseudomonas fluorescens
• recombinant: expressed in E. coli
• Assay: 0.5—2.0 mg protein/mL (biuret)
• form: ammonium sulfate suspension
• specific activity: ≥50 units/mg protein (biuret)
• color: white
• suitability: suitable for enzyme test
• application(s): life science and biopharma
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: Pseudomonas fluorescens ... gdh(533113295)

Description

Application

β-Galactose Dehydrogenase from Pseudomonas fluorescens has been used for competitive inhibition in lectin histochemistry. It has also been used to measure the hydrolysis activity of Haloferax alicantei β-galactosidase on different disaccharides.

Biochem/physiol Actions

β-galactose dehydrogenase catalyzes the oxidation of β-D-galactose to D-galactono-gammalactone.

Unit Definition

One unit will convert 1.0 μmole of D-galactose to D-galactonate per min at pH 8.6 at 25 °C.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, pH approx. 6.0

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves