F7378

Fluorescein diacetate

Powder

• Synonym(s): 3,6-Diacetoxyfluoran, Di-O-acetylfluorescein
• Empirical Formula (Hill Notation): C₂₄H₁₆O₇
• CAS Number: 596-09-8
• Molecular Weight: 416.38
• Beilstein: 62575
• EC Number: 209-877-6
• MDL number: MFCD00005062
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894939
• NACRES: NA.47

Properties

• product name: Fluorescein diacetate, used as cell viability stain
• form: powder
• color: white to faint yellow
• mp: 200-203 °C (lit.)
• solubility: acetone: 25 mg/mL
• ε (extinction coefficient): ≥86000 at 488-494 nm in 0.1 M NaOH
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1
• InChI: 1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
• InChI key: CHADEQDQBURGHL-UHFFFAOYSA-N

Description

General description

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.

Application

Fluorescein diacetate has been used for the differentiation of live cells from dead cells.

Substrates

Lipase substrate

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves