F2637
Ficoll® 400
BioXtra, for molecular biology, lyophilized powder
Synonym(s):
Polysucrose 400, Ficoll 400
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About This Item
Recommended Products
biological source
non-animal source
Quality Level
grade
Molecular Biology
for molecular biology
product line
BioXtra
form
lyophilized powder
foreign activity
DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected
storage temp.
room temp
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General description
Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.
Application
Suitable for
- hybridization experiments to reduce non-specific binding of labeled probes to the membrane
- preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
- separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
- producing density gradients to separate subcellular components
- vitrification of cell and tissue samples.
- to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).
Used for cell separation and organelle isolation.
Features and Benefits
- free from DNases, RNases, phosphatases and proteases
- preserves functional and morphological integrity of the separated cells
Other Notes
A nonionic synthetic polymer of sucrose.
Legal Information
Ficoll is a registered trademark of Cytiva
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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