F1763

Anti-Rat IgG (whole molecule)–FITC antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Rabbit Anti-Rat IgG (whole molecule)–Fluorescein isothiocyanate
• MDL number: MFCD00162811
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• conjugate: FITC conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• storage condition: protect from light
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320; indirect immunofluorescence: 1:320
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Anti-Rat IgG (whole molecule) is produced in rabbit using purified rat IgG as the immunogen.

Rat IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rat serum. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Anti-Rat IgG (whole molecule)-FITC antibody binds to all rat immunoglobulins.

Specificity

Rabbit polyclonal anti-Rat IgG (whole molecule)−FITC antibody reacts with rat IgG in vitro, in rat serum and biological fluids.

Immunogen

Purified rat IgG

Application

Anti-Rat IgG (whole molecule)-FITC antibody has been used:

• in immunolabelling for confocal laser scanning microscopy (CLSM)
• in immunostaining

Anti-Rat IgG (whole molecule)-FITC antibody is suitable for use in flow cytometry (1:100) and immunofluorescence.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)

Rabbit polyclonal anti-Rat IgG (whole molecule)-FITC antibody may be used to detect and quantitate the level of IgG in rat serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use pig IgG as the primary antibody.

Biochem/physiol Actions

IgGs are glycoprotein antibodies that modulate several immune responses. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves