D5540
Diaphorase from Clostridium kluyveri
lyophilized powder, 3.0-20.0 units/mg protein (biuret)
Synonym(s):
Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase
About This Item
Recommended Products
biological source
bacterial (Clostridium kluyveri)
Quality Level
form
lyophilized powder
specific activity
3.0-20.0 units/mg protein (biuret)
shipped in
wet ice
storage temp.
−20°C
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This Item | D2197 | K1502 | SAE0049 |
---|---|---|---|
3.0-20.0 units/mg protein (biuret) | >=30 units/mg protein (biuret) | 0.1-1.0 units/mg protein (Lowry) | - |
lyophilized powder | solid | buffered aqueous glycerol solution | aqueous solution |
wet ice | - | dry ice | dry ice |
−20°C | −20°C | −20°C | −20°C |
200 | 200 | 200 | 200 |
Application
Packaging
Other Notes
Diaphorases which are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities. It is reported to be a single protein. However, Massey reports that "diaphorase" is probably a denatured lipoamide dehydrogenase. Pre-incubation of the pig heart preparation with Cu2+ reduces the lipoamide dehydrogenase activity and proportionately increases the β-NADH diaphorase activity. In our laboratory, we have demonstrated this copper effect to some degree on the pig heart enzyme, but no appreciable effect was observed on the Clostridium kluyveri or torula yeast preparations. The lipoamide dehydrogenase:diaphorase ratio is a measure of the denaturation.
Unit Definition
inhibitor
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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