C2C12 Cell Line from mouse
91031101, C3H muscle myoblast
Synonym(s):
C2-C12 Cells
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About This Item
UNSPSC Code:
41106514
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biological source
mouse muscle
growth mode
Adherent
karyotype
Not specified
morphology
Myoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse C3H muscle myoblast
Cell Line Description
Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.
Application
C2C12 Cell Line has been used to study the the expression of OXT and OXTR (oxytocin and its receptor) genes and proteins and in differentiation studies.
Subculture Routine
Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C. Do not allow cells to reach confluence.
Other Notes
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Certificates of Analysis (COA)
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