CHP 4 (W.W.)

90102537, human skin, Fibroblast

• UNSPSC Code: 41106514

Properties

• product name: CHP 4 (W.W.), 90102537
• biological source: human skin
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human skin asymptomatic Galactosemia

Cell Line Description

Derived from skin tissue taken from an 8 year old male with asymptomatic galactosemia, despite lacking red cell galactose-1-phosphate uridyl transferase. Sibling of CHP 3 (M.W.) (Sigma catalogue no. 90102536) with the same enzyme deficiency that was symptomatic. Cultures will retain characteristics for at least 30 passages. Ethnicity: Black.

Application

Enzyme deficiency

Culture Medium

DMEM + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

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