AFRC MAC 14
86030303, Reactivity: Anti-Glycoprotein of Chlamydomonas reinhardii
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About This Item
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biological source
Rat x Rat Hybridoma
ImmunoDon
AO rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-Glycoprotein of Chlamydomonas reinhardii
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Y3Ag1.2.3 x Rat AO spleen
Cell Line Description
The antibody recognises the glycoprotein 2BII. Binding is lost after acid hydrolysis or de-glycosylation of 2BII. AFRC MAC 14 shows similar fine specificity and properties to AFRC MAC 12, 13, 15, 16 and 17.
Immunogen
2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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