LIM1899 cell line human
12062001
Synonym(s):
LIM 1899, LIM-1899
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About This Item
UNSPSC Code:
41106514
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biological source
human colon
growth mode
adherent
morphology
Epithelial; cells are small rounded and grow in clusters
shipped in
dry ice
storage temp.
−196°C
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Cell Line Description
Columnar cell adenocarcinoma of the colon. Cell Bank Australia reference number: CBA0163.
Culture Medium
RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM
Subculture Routine
Optimal split ratio 1:8 (seeding density 1 x 104 cells/cm2). Cells incubated at 37°C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. LIM1899 requires the growth medium to be changed 3 times each week.
Other Notes
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Certificates of Analysis (COA)
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