Sf21

5022801, insect ovary, Spherical

• UNSPSC Code: 41106514

Properties

• product name: Sf21, 05022801
• biological source: insect ovary
• growth mode: Adherent
• karyotype: Not specified
• morphology: Spherical
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Spodoptera frugiperda pupal ovarian tissue

Cell Line Description

Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems. They were derived at the USDA Insect Pathology Laboratory from a cell line originating from pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Sf21 cells have greater range in size and have a doubling time of 24 hr compared to 48-72 hr for Sf9 cells.

Application

Protein expression using the Baculovirus vector system

Culture Medium

SF90011 + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x10,000 cells/cm²; 28 C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, centrifuge suspension and resuspend cell pellet in fresh medium.

Other Notes

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