CASP3C

Caspase 3 Assay Kit, Colorimetric

• Synonym(s): Colorimetric Caspase-3 Activity Assay Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 1,000 multiwell tests (in 96-well multiwell plates); sufficient for 100 standard tests (1 ml)
• Quality Level: 300
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CASP3(836)

Description

General description

Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.

The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (ε_mM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).

Application

The assay can be performed (a) in 1 mL volume and measured using a spectrophotometer, or (b) in 100 µL volume in a 96-well plate using an ELISA reader.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.

Other Notes

Use 17 megohm water only

Kit Components Only

• Water (17-18 megaOhm) 125 mL

Kit Components Also Available Separately

• A0835 Ac-DEVD-CHO inhibitor .5 mg
• A2559 Ac-DEVD-pNA substrate 15 mg
• p-Nitroaniline standard 1 mg

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H301 + H311 + H331,H373,H412
• Precautionary Statements: P273 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P311 - P314
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Flash Point(F): 415.4 °F - closed cup
• Flash Point(C): 213 °C - closed cup