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Key Documents

C167

Sigma-Aldrich

Cholera Toxin B subunit

solid

Synonym(s):

CTB

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About This Item

MDL number:
MFCD02356522
UNSPSC Code:
12352200
PubChem Substance ID:
329774903
NACRES:
NA.77

form

solid

Quality Level

200

mol wt

~12 kDa

color

white

storage temp.

2-8°C

SMILES string

CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F

InChI

1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)

InChI key

YDXZSNHARVUYNM-UHFFFAOYSA-N

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General description

Cholera Toxin B subunit is secreted by Vibrio cholerae. It is a pentamer and is nontoxic in nature.

Application

Cholera Toxin B subunit has been used as a tracer to study the ascending projections of the nuclei of the descending trigeminal tract (nTTD) in the zebra finch.

Biochem/physiol Actions

Cholera Toxin B subunit stimulates both systemic and mucosal antibody production in animals and humans. CTB functions as an oral subunit vaccine for cholera, which is associated with acute watery diarrhoea.
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Caution

Do not freeze.

Reconstitution

When reconstituted in 0.25 mL distilled water, the compound contains 500 μg of protein in 0.01 M sodium phosphate at pH 7.5.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Efficacy of a food plant-based oral cholera toxin B subunit vaccine
Arakawa T, et al.
Nature Biotechnology, 16(3), 292-292 (1998)
J Holmgren et al.
Journal of general microbiology, 86(1), 49-65 (1975-01-01)
Structural analysis of cholera toxin by sodium dodecylsulphate polyacrylamide electrophoresis demonstrated two types of non-covalentyly linked subunits, heavy (H) AND LIGHT (L), with respective molecular weights 28000 and 800 to 9000. The H:L protein ratio was I:2, indicating that the
Gangliosides as membrane receptors for tetanus toxincholera toxin and serotonin.
van Heyningen, et al.
Nature, 249, 415-417 (1974)
The ascending projections of the nuclei of the descending trigeminal tract (nTTD) in the zebra finch (Taeniopygia guttata)
Faunes M, et al.
The Journal of Comparative Neurology, 525(5), 2832-2846 (2017)
Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts1
Daniell H, et al.
Journal of Molecular Biology, 311(5), 1001-1009 (2001)
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