Skip to Content
Merck
All Photos(1)

Documents

B0519

Sigma-Aldrich

Biotin−Agarose

PBS suspension

Synonym(s):

Vitamin H Agarose

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00163072
UNSPSC Code:
41106500
NACRES:
NA.56

form

PBS suspension

Quality Level

200

extent of labeling

≥30 mg avidin per mL packed resin
2-6 μmol per mL

matrix

4% beaded agarose

matrix activation

epoxy

matrix attachment

carboxy (amide linkage); resin remains relatively uncharged over wide pH range

matrix spacer

13 atoms

application(s)

life science and biopharma

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Biotin contains a high affinity for binding to avidin or streptavidin. It has been widely used in biochemistry and molecular biology for detecting and labeling proteins.

Physical form

Suspension in phosphate buffered saline containing 0.02% sodium azide

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

K J Airenne et al.
Gene, 144(1), 75-80 (1994-06-24)
A recombinant avidin (re-Avd), containing amino acids (aa) 1-123 of the native chicken egg-white Avd, was produced in Escherichia coli. When cells were grown at 37 degrees C production was over 1 microgram/ml, due to altering the codon preference of
A P Sibler et al.
Journal of immunological methods, 224(1-2), 129-140 (1999-06-05)
In vivo biotinylation of antibody fragments with a gene fusion approach is a realistic alternative to conventional in vitro chemical labelling. We have previously reported the construction of a vector system suitable for the bacterial expression of the binding fragment
Emrah Celik et al.
Journal of molecular recognition : JMR, 25(1), 53-56 (2012-01-04)
Sample-probe contact duration (dwell time) and loading force are two important parameters for the atomic force microscopy (AFM) force spectroscopy measurements of ligand-receptor interaction. A prolonged contact time may be required to initiate ligand-receptor binding as a result of slow
P A Smith et al.
Nucleic acids research, 26(6), 1414-1420 (1998-04-29)
The high affinity binding interaction of biotin to avidin or streptavidin has been used widely in biochemistry and molecular biology, often in sensitive protein detection or protein capture applications. However, in vitro chemical techniques for protein biotinylation are not always
Biotinylated CdSe/ZnSe nanocrystals for specific fluorescent labeling.
Charvet, N., et al.
Journal of Materials Chemistry, 14, 2638-2642 (2004)
View All Related Papers

Related Content

Protein Pull-Down

Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service