A4675

Agar

Type E, suitable for plant cell culture

• Synonym(s): Agar-agar, Gum agar
• Linear Formula: (C₁₂H₁₈O₉)_n
• CAS Number: 9002-18-0
• EC Number: 232-658-1
• MDL number: MFCD00081288
• UNSPSC Code: 50405901
• eCl@ss: 42040102
• NACRES: NA.21

Properties

• biological source: algae (Rhodophyceae)
• Quality Level: 200
• type: Type E
• form: powder
• technique(s): cell culture | plant: suitable
• transition temp: transition temp 32-38 °C (1.5%)
• application(s): agriculture
• storage temp.: room temp
• InChI: 1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1
• InChI key: GYYDPBCUIJTIBM-DYOGSRDZSA-N

Description

General description

Agar or Type E agar is a thermo-reversible, polysaccharide gelling agent. Structurally it consists of two main fractions about 70% of agarose and 30% of agaropectin. The gelling properties of agar comes from the agarose, the high gel strength backbone consists of a linear polymer of 3-linked β-D-galactose and 4-linked 3,6-anhydro-α-L-galactose (LA) repeating units.

Agar or Type E agar is a thermo-reversible, polysaccharide gelling agent. Structurally, it consists of two main fractions about 70% of agarose and 30% of agaropectin. The gelling properties of agar comes from the agarose, the high gel strength backbone consists of a linear polymer of 3-linked ß-D-galactose and 4-linked 3,6-anhydro-α-L-galactose (LA) repeating units.

Application

Agar has been used as reagent for the following studies:

• Plant cell culture studies.
• As medium supplement for the growth of wild-type Arabidopsis thaliana (ecotype Columbia) and transgenic seeds.
• For the solidification of culture medium of cork oak somatic embryos.
• to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants

Agar suspension culture may be used for the analysis of cell transformation of palyoma virus. It might have been used for incubation of single antibody-forming cell to investigate the plaque formation.

Agar has been used to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves