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Sigma-Aldrich

Anti-Rabbit IgG F(ab′)2 fragment - Atto 488, whole molecule antibody produced in goat

1 mg/mL protein

Synonym(s):

Atto 488-[F(ab′)2 fragment goat-Anti-rabbit IgG], whole molecule

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

conjugate

Atto 488 conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

contains

50% glycerol as stabilizer

species reactivity

rabbit

concentration

1 mg/mL protein

fluorescence

λex 500 nm; λem 522 nm in PBS

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-rabbit IgGs associate with rabbit IgGs.

Immunogen

rabbit IgG F(ab′)2 fragment

Application

Atto 488-goat anti-rabbit IgG F(ab′)2 fragment, whole molecule antibody can be used for fluorescence-based immunoassays.

Physical form

Atto 488 F(ab)′2 fragment goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1mg/mL solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Preparation Note

F(ab)′2 fragment goat anti-rabbit is conjugated to Atto 488 NHS and purified by gel permeation chromatography and dialysis to remove unbound dye.

Analysis Note

Unconjugated dye ≤5% of total fluorescence; dye-to protein ratio ≥2

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The changing point-spread function. Single-molecule-based super-resolution imaging.
Horrocks, M. H.; Palayret, M.; Klenerman, D.; Lee, S. F.
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Ullal, Chaitanya K.; et al.
Macromolecules, 44(19), 7508-7510 (2011)
Super-Resolution Fluorescence Microscopy.
Huang, B.; Bates, M.; Zhuang, X.
Annual Review of Biochemistry, 78, 993-1016 (2009)
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Jungmann, R.; Avendano, M.
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Staudt, T.; et al.
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