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34860

Sigma-Aldrich

Methanol

≥99.9%, suitable for immunofluorescence, HPLC

Synonym(s):

Methyl alcohol

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About This Item

Linear Formula:
CH3OH
CAS Number:
Molecular Weight:
32.04
Beilstein:
1098229
EC Number:
MDL number:
UNSPSC Code:
41116105
PubChem Substance ID:
NACRES:
NA.21

product name

Methanol, suitable for HPLC, ≥99.9%

vapor density

1.11 (vs air)

Quality Level

vapor pressure

410 mmHg ( 50 °C)
97.68 mmHg ( 20 °C)

Assay

≥99.9%

form

liquid

autoignition temp.

725 °F

expl. lim.

36 %

technique(s)

HPLC: suitable
immunofluorescence: suitable

impurities

≤0.0005% non-volatile matter
≤1 ppb fluorescence (quinine) at 254 nm
≤1 ppb fluorescence (quinine) at 365 nm
<0.03% water (Karl Fischer)

evapn. residue

<0.0005%

color

APHA: ≤10

refractive index

n20/D 1.329 (lit.)

bp

64.7 °C (lit.)

mp

−98 °C (lit.)

density

0.791 g/mL at 25 °C (lit.)

λ

neat

UV absorption

λ: 205 nm Amax: ≤1.00
λ: 210 nm Amax: ≤0.60
λ: 220 nm Amax: ≤0.30
λ: 230 nm Amax: ≤0.20
λ: 235 nm Amax: ≤0.10
λ: 240 nm Amax: ≤0.10
λ: 260 nm Amax: ≤0.04
λ: 280 nm Amax: ≤0.01
λ: 400 nm Amax: ≤0.01

application(s)

food and beverages

format

neat

SMILES string

CO

InChI

1S/CH4O/c1-2/h2H,1H3

InChI key

OKKJLVBELUTLKV-UHFFFAOYSA-N

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General description

Methanol is an organic solvent that can be synthesized from syngas in the presence of CuO/ZnO/Al2O3 catalysts. It is an ideal candidate as a hydrogen source in fuel cell technology due to its high H/C ratio, low propensity for soot generation, relatively low reforming temperature and as it exists in liquid state at room temperature. In a direct methanol fuel cell (DMFC), methanol undergoes oxidation with air to generate electricity. The olefins (ethylene or propylene) formed from methanol via MTO (methanol-to-olefins) process, can be an alternate to oil and gas to produce hydrocarbon fuels.
Thermochemical conversion of methanol to C2-C10 hydrocarbons in the presence of shape-selective zeolites has been reported. Its oxidation on Ru-Pt catalyst system by ruthenium ad-atoms has been proposed.

Application

Methanol (MeOH) has been used in the wholemount immunofluorescence studies of embryonic tissues.
It may be used in the following studies:
  • Colony forming unit-fibroblast assay of bone marrow mononuclear cells.
  • As solvent for the preparation of extracts of hyphae of Aspergillus for the estimation of gliotoxin by reversed phase-HPLC.
  • Immunofluorescence studies.
  • To compose the eluent for the ion-pair reverse-phase HPLC isolation of nucleotides and their decomposition products.
Methanol can be used as a mobile phase in reversed-phase liquid chromatography.

Preparation Note

Product filtered through a 0.2 μm filter

Other Notes

Important notice
  • The article number 34860-4X2.5L-R will be discontinued. Please order the single bottle 34860-2.5L-R which is physically identical with the same exact specifications.
  • The article number 34860-6X1L-R will be discontinued. Please order the single bottle 34860-1L-R which is physically identical with the same exact specifications.

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Legal Information

Pure-Pac is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

49.5 °F - closed cup

Flash Point(C)

9.7 °C - closed cup


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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High-performance liquid chromatography of thiols and disulfides: dinitrophenol derivatives.
Fariss MW and Reed DJ.
Methods in Enzymology, 143, 101-109 (1987)
Jonas Ahnfelt-Rønne et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 55(9), 925-930 (2007-05-05)
We have developed a wholemount immunofluorescence protocol for the simultaneous detection of up to three proteins in mouse and chicken embryos. Combined with Murray's clearing reagent (BABB) and microscope objectives with long working ranges and high numerical apertures mounted on
Mehmet Nuri Atalar et al.
Life sciences, 231, 116577-116577 (2019-06-19)
Galectin-3 is associated with the process of inflammation and fibrosis. The aim of this study was both to evaluate of galectin-3, methylated arginines and hs-CRP in subjects with type 2 diabetes and prediabetes and to investigate a relation between serum
Beyond oil and gas: the methanol economy.
Olah GA.
Angewandte Chemie (International Edition in English), 44(18), 2636-2639 (2005)
Catalytic carbon dioxide hydrogenation to methanol: A review of recent studies.
Jadhav SG, et al.
Chemical Engineering Research and Design, 92(11), 2557-2567 (2014)

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Protocols

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry

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