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13767

Supelco

Silica gel orange

with moisture indicator free of heavy metals

Synonym(s):

Silica gel

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352309
PubChem Substance ID:

quality

with moisture indicator free of heavy metals

impurities

≤0.025% soluble in chloroform

loss

≤2% loss on drying, 150 °C, 3 h

particle size

2-5 mm

capacity

≥25 % absorption capacity (water)

bp

2230 °C

mp

>1600 °C

SMILES string

O=[Si]=O

InChI

1S/O2Si/c1-3-2

InChI key

VYPSYNLAJGMNEJ-UHFFFAOYSA-N

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General description

Silica gel orange (Drying pearls Orange) is a commonly employed drying agent.

Application

Silica gel orange (Drying pearls Orange) may be used to provide long-term storage conditions in the following studies:
  • Multiwell plates in a gel drying box during the solid-phase reverse transfection on cell microarrays.
  • Robust protocol for the reverse transfection of cells on small interfering (siRNA) arrays.
It was used to compose the spotting solution of transfection array.

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Holger Erfle et al.
Journal of biomolecular screening, 13(7), 575-580 (2008-07-05)
Solid-phase reverse transfection on cell microarrays is a high-throughput method for the parallel transfection of mammalian cells. However, the cells transfected in this way have been restricted so far to microscopy-based analyses. Analysis methods such as reverse transcriptase-polymerase chain reaction
Holger Erfle et al.
Methods in molecular biology (Clifton, N.J.), 785, 277-287 (2011-09-09)
Endocytosis is one of the most essential cellular processes, which enables cells to internalise diverse -material. It is crucial for regulation of receptor activity and signalling, cell polarisation, attachment and motility, and a great number of other cellular functions. A
Holger Erfle et al.
Nature protocols, 2(2), 392-399 (2007-04-05)
Here, we describe a robust protocol for the reverse transfection of cells on small interfering (siRNA) arrays, which, in combination with multi-channel immunofluorescence or time-lapse microscopy, is suitable for genome-wide RNA interference (RNAi) screens in intact human cells. The automatic
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