68109

Nickels and Leesment ChromoSelect Medium

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Nickels and Leesment Medium + X-gal
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15.0 g/L ; calcium lactate pentahydrate, 8.0 g/L ; carboxymethyl cellulose (CMC), 0.40 g/L ; casein enzymic hdrolysate, 18.0 g/L ; chromogenic substrate (X-gal), 0.20 g/L ; gelatine, 2.25 g/L ; glucose, 4.5 g/L ; lactose, 4.5 g/L ; sodium chloride, 3.6 g/L ; tricalcium dicitrate tetrahydrate, 6.65 g/L ; trisodium citrate dihydrate, 1.8 g/L ; yeast extract, 4.5 g/L
• manufacturer/tradename: NutriSelect^® Plus
• final pH: 6.65±0.05 (25 °C)
• application(s): bioburden testing; environmental; food and beverages; microbiology
• storage temp.: 2-8°C
• suitability: Lactococcus spp.; Leuconostoc spp.

Description

Application

Nickels and Leesment ChromoSelect Medium is used for the enumeration of citrate-fermenting lactic acid bacteria from milk, milk products and mesophilic starter cultures.

Preparation Note

Supplement:
2 vl/L of Nickels and Leesment ChromoSelect Selective Supplement (Cat. No. 40952).

Suspend 66.0 grams of dehydrated medium in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, add rehydrated contents of 2 vials of Nickels and Leesment ChromoSelect Selective Supplement (Cat. No. 40952).

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable