MABS2219
Anti-MGAT1 Antibody, clone 5F5
Synonym(s):
Anti-MGAT1 Antibody, clone 5F5
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
5F5, monoclonal
mol wt
calculated mol wt 38.79 kDa
observed mol wt ~36 kDa
purified by
using protein G
species reactivity
mouse
packaging
antibody small pack of 100 μg
technique(s)
western blot: suitable
isotype
IgG2κ
epitope sequence
C-terminal half
Protein ID accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
mouse ... Mogat1(68393)
General description
2-acylglycerol O-acyltransferase 1 (UniProt: EC:2.3.1.22, Acyl-CoA:monoacylglycerol acyltransferase 1, MGAT1,Diacylglycerol acyltransferase 2-like protein 1, Monoacylglycerol O-acyltransferase 1, MGAT1) is encoded by the Moghat1 (also known as Dgat2l1) gene (Gene ID: 68393) in murine species. MGAT1 is an enzyme that catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Its activity is reported to be directly regulated by PPARg and it plays a role in regulating the pathway that leads to incorporation of fatty acids into triglycerides. MGAT1 activity is shown to be higher in mice fed high fat diet and by overexpression of PPARg, which leads to hepatic steatosis. Even in cultured hepatocytes, overexpression of MGAT1 is shown to increase triglyceride synthesis without affecting the expression of PPARg. The binding of PPARg to MGAT1 promoter is significantly enhanced Ob/Ob and high fat diet fed mice. MGAT1 expression is significantly reduced in PPARg knockdown murine models and knockdown of MGAT1 expression is shown to improve hepatic steatosis in high fat fed mice. It has also been shown that C3H mice that do not express PPARg and MGAT1 in the liver do not develop hepatic steatosis after being fed a high fat diet. (Ref.: Lee, YJ., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(34); 13656-13661).
Specificity
Clone 5F5 is a mouse monoclonal antibody that detects Monoacylglycerol O-acyltransferase 1 (MGAT1). It targets an epitope within 13 amino acids from the C-terminal half.
Immunogen
KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal half of murine 2-acylglycerol O-acyltransferase 1 (MGAT1).
Application
Quality Control Testing
Evaluated by Western Blotting in lysate from HEK293 cells overexpressing MGAT1.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected MGAT1 in lysates from HEK293 cells transfected with mouse MGAT1, but not in untransfected cells.
Tested Applications
Western Blotting Analysis: A representative lot detected MGAT1 in Western Blotting applications (Lee, Y.J., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(34):13656-61).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysate from HEK293 cells overexpressing MGAT1.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected MGAT1 in lysates from HEK293 cells transfected with mouse MGAT1, but not in untransfected cells.
Tested Applications
Western Blotting Analysis: A representative lot detected MGAT1 in Western Blotting applications (Lee, Y.J., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(34):13656-61).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-MGAT1, clone 5F5, Cat. No. MABS2219, is a mouse monoclonal antibody that detects 2-acylglycerol O-acyltransferase 1 (MGAT1) and is tested for use in Western Blotting,
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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