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Key Documents

MAB2146Z

Sigma-Aldrich

Anti-ICAM-1 Antibody, clone P2A4, Azide Free

clone P2A4, Chemicon®, from mouse

Synonym(s):

CD54, MAB2146

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

P2A4, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ICAM1(3383)

Specificity

Human ICAM-1

Immunogen

IL-1beta activated HUVEC (human)

Application

Immunohistochemistry: for use on acetone or paraformaldehyde fixed tissue

Immunocytochemistry: for use on B cells, PBL, T cells

Immunoprecipitation

FACS

ELISA

Inhibits ICAM-1 dependent adhesive interactions

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-ICAM-1 Antibody, clone P2A4, Azide Free is validated for use in ELISA, FC, IP, IC, IH, FUNC for the detection of ICAM-1.

Physical form

Format: Purified
Sterile-filtered lquid containing 0.2M phosphate buffer, 0.25M sodium chloride, no preservatives.

Storage and Stability

Maintain at -20°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro.
Ghislin, S; Obino, D; Middendorp, S; Boggetto, N; Alcaide-Loridan, C; Deshayes, F
BMC Cancer null
Protection of CLL B cells by a follicular dendritic cell line is dependent on induction of Mcl-1.
Irene M Pedersen, Shinichi Kitada, Lorenzo M Leoni, Juan M Zapata, James G Karras et al.
Blood null
Evaluation of the sclerotherapeutic efficacy of ethanol, polidocanol, and OK-432 using an in vitro model.
William Mol, Hiroshi Furukawa, Satoru Sasaki, Utano Tomaru, Toshihiko Hayashi et al.
Dermatologic Surgery : Official Publication For American Society For Dermatologic Surgery [Et Al.] null
Lymphocyte function-associated antigen-1-mediated T cell adhesion is impaired by low molecular weight phosphotyrosine phosphatase-dependent inhibition of FAK activity.
Giannoni, E; Chiarugi, P; Cozzi, G; Magnelli, L; Taddei, ML; Fiaschi, T; Buricchi et al.
The Journal of Biological Chemistry null
Roles of phosphatidylinositol 3-kinase and NF-kappaB in human cytomegalovirus-mediated monocyte diapedesis and adhesion: strategy for viral persistence.
Smith, MS; Bivins-Smith, ER; Tilley, AM; Bentz, GL; Chan, G; Minard, J; Yurochko, AD
Journal of virology null

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