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Key Documents

AB6020

Sigma-Aldrich

Anti-MAP4 Antibody

from rabbit

Synonym(s):

microtubule-associated protein 4, cdc kinase, MAP-4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, horse, mouse, rat

species reactivity (predicted by homology)

equine (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

NP_002366

UniProt accession no.

P27816

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MAP4(4134)

General description

Microtubule-associated protein 4 (MAP4) is a ubiquitous non-neuronal microtubule-associated protein. This protein binds, polymerizes, and stabilizes microtubules and is thought to regulate microtubule dynamics during the cell cycle. p34cdc2 kinase phosphorylation of MAP4 appears to regulate the control of microtubule reorganization at mitosis (Ookata, 1997).

Specificity

Antibody recognizes the C-terminus of MAP4.

Immunogen

KLH-conjugated peptide corresponding to the C-terminus of human MAP4.

Application

This Anti-MAP4 Antibody is validated for use in WB, IC for the detection of MAP4.
Western blot (SNAP ID) Analysis: 0.5 µg/mL from a previous lot detected MAP4 in 10 µg of HeLa cell lysate.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected MAP4 in HeLa, NIH/3T3, and A431 cells.

Quality

Evaluated by Western blot in HeLa cell lysate.

Western blot Analysis: 0.05 µg/mL of this antibody detected MAP4 in 10 µg of HeLa cell lysate.

Target description

~ 220 kDa

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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