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Key Documents

524545

Sigma-Aldrich

Phosphatase, Alkaline, E. coli

Synonym(s):

Phosphatase, Alkaline, E. coli

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352202

form

liquid

Quality Level

specific activity

≥500 units/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

pI 

4.5

foreign activity

Non-specific endonuclease, RNase, none detected

shipped in

wet ice

storage temp.

−20°C

General description

Native alkaline phosphatase from E. coli. A dimeric zinc- and magnesium-containing protein. A non-specific phosphomonoesterase with a pI of 7.5.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of pNPP per min at 25°C in Tris-HCl, pH 8.0.

Physical form

In 50 mM Tris-HCl, 50% glycerol, pH 7.5.

Other Notes

Reid, T.W. and Willson, I.B. 1971. Enzymes4, 383.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

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