411492
Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025)
liquid, clone HP6025, Calbiochem®
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
secondary antibodies
clone
HP6025, monoclonal
form
liquid
does not contain
preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Purified mouse monoclonal antibody. Recognizes human IgG4 Fc fragment.
This Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025) is validated for use in Enzyme Immunoassay for the detection of Human IgG₄, Fc Fragment Specific.
Application
Enzyme Immunoassay (10 µg/ml, see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, pH 7.4.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
Other Notes
Antibody should be titrated for optimal results in individual systems.
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: ≤0.1%
Human IgG2: ≤0.04%
Human IgG3: ≤0.02%
Human IgG4: 100%
Human IgA: <0.1%
Human IgE: <0.1%
Human IgM: <0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: <0.01%
Human IgG2: <0.01%
Human IgG3: <0.01%
Human IgG4: 100%
Human IgA: <0.01%
Human IgE: <0.01%
Human IgM: <0.01%
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: ≤0.1%
Human IgG2: ≤0.04%
Human IgG3: ≤0.02%
Human IgG4: 100%
Human IgA: <0.1%
Human IgE: <0.1%
Human IgM: <0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: <0.01%
Human IgG2: <0.01%
Human IgG3: <0.01%
Human IgG4: 100%
Human IgA: <0.01%
Human IgE: <0.01%
Human IgM: <0.01%
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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D C Houpt et al.
Infection and immunity, 62(7), 2857-2864 (1994-07-01)
Serum obtained from normal human subjects contains antibodies reactive in an enzyme-linked immunosorbent assay with the glucuronoxylomannan (GXM) of Cryptococcus neoformans. The frequency of occurrence of class-specific antibodies among normal subjects was 28% for immunoglobulin G (IgG), 98% for IgM
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