251760
DAR-4M
A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.
Synonym(s):
DAR-4M, Diaminorhodamine-4M
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About This Item
Empirical Formula (Hill Notation):
C25H26N4O3
Molecular Weight:
430.50
UNSPSC Code:
12352202
NACRES:
NA.25
Quality Level
Assay
≥98% (HPLC)
form
solution
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
red-violet
fluorescence
λex ~560 nm
λem ~575 nm
shipped in
ambient
storage temp.
−20°C
General description
A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500).
A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Unlike DAF-2 (Cat. No. 251500), its fluorescence intensity is not dependent on the pH. Excitation maximum: ~560 nm; emission maximum: ~575 nm.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Nitric oxide (NO) fluorescent indicator
Nitric oxide (NO) fluorescent indicator
Product does not compete with ATP.
Reversible: no
Warning
Toxicity: Irritant (B)
Physical form
In DMSO.
Reconstitution
Following initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.
Other Notes
Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.
Legal Information
Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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