1.25139

Fractogel^® EMD SO₃ ⁻ (M) Resin

RoboColumn Fractogel^® SO3 (M), volume 0.6 mL, prepacked column

• Synonym(s): RoboColumn Fractogel^® SO3 (M), 0.6 ml
• UNSPSC Code: 41115711

Properties

• ligand: (Sulfoisobutyl)
• Quality Level: 100
• material: polypropylene housing
• product line: Fractogel^®
• form: prepacked column
• parameter: ≤8 bar pressure; ~200 cm/hr max. flow rate
• column I.D. × L: 5 mm × 30 mm
• volume: 0.6 mL
• matrix active group: Methacrylate
• mean particle size: 40-90 μm
• capacity: ~130 mL, resin binding capacity
• application(s): mAb; recombinant protein; vaccine development
• separation technique: cation exchange
• storage temp.: 15-25°C

Description

General description

5 x 30 mm

Features and Benefits

RoboColumn Fractogel^® SO3 (M), 0.6 ml enables:

• Increased binding strength at low pH
• High aggregate removal efficiency combined with high dynamic binding capacity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel^® EMD SO₃^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany