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1.00432

Millipore

ChromoCult® Listeria Agar Selective-Supplement

for Listeria spp., pkg of 10 vials, for the preparation of ChromoCult® Listeria agar (base)

Synonym(s):

Listeria Selective Supplement

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About This Item

UNSPSC Code:
41106212

Agency

ISO 11290

Quality Level

100

form

lyophilized powder

composition

Amphotericin B, 5 mg per vial
Ceftazidime, 10 mg per vial
Nalidixic acid sodium salt, 10 mg per vial
Polymyxin B sulphate, 38350 (U per vial)

packaging

pkg of 10 vials

technique(s)

microbiological culture: suitable

pH

6.2 (20 °C in H2O, Aqueous solution)

application(s)

food and beverages
microbiology

compatibility

ChromoCult® Listeria agar (base) acc. OTTAVIANI and AGOSTI (1.00427)

storage temp.

2-8°C

suitability

Listeria spp.

General description

ChromoCult® Listeria Agar Selective-Supplement contains a mixture of four antibiotics in lyophilized form. It largely inhibits the growth of accompanying bacteria by selective enrichment of Listeria spp. Amphotericin B reduces the growth of yeasts and molds. Polymyxin B inhibits the growth of Gram-negative bacteria. While nalidixic acid sodium salt mainly inhibits the growth of Gram-negative bacteria, it also inhibits some Gram-positive bacteria. Ceftazidime has a broad-spectrum activity against both Gram-negative and Gram-positive bacteria.

Application

FRASER Listeria Selective Supplement is an additive for the preparation of ChromoCult® Listeria agar (base) for the selective growth of Listeria spp.

Linkage

Replaces: 1.00432.0017

Analysis Note

Growth promotion test according to ISO 11133.
Inoculum on reference medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
10 - 100
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100
- Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100
Recovery on test medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
≥ 70 %
- Listeria monocytogenes ATCC 19111 (WDCM 00020): ≥ 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %
Colonies
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
blue-green; non-transparent zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone
- Listeria innocua ATCC 33090 (WDCM 00017): blue-green
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): no growth
Incubation: Listeria 24-48 hrs. 37°C aerobic; other strains at least 48 hrs.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317 - H334 - H351 - H373

Hazard Classifications

Carc. 2 - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

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Articles

Detection of Listeria monocytogenes in Food and Environmental Samples

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

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