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Key Documents

05-538

Sigma-Aldrich

Anti-phospho-Raf-1 (Ser338) Antibody

Upstate®, from mouse

Synonym(s):

Anti-CMD1NN, Anti-CRAF, Anti-NS5, Anti-Raf-1, Anti-c-Raf

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

monoclonal

species reactivity

mouse

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_002880

UniProt accession no.

P04049

shipped in

wet ice

target post-translational modification

phosphorylation (pSer338)

Gene Information

human ... RAF1(5894)

Specificity

Recognizes Raf-1 phosphorylated at Serine 338.

Immunogen

Peptide corresponding to residues 333-345 of human Raf-1

Application

Anti-phospho-Raf-1 (Ser338) Antibody detects level of phospho-Raf-1 (Ser338) & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
MAP Kinases

Quality

routinely evaluated by immunoblot on RIPA lysates from 3T3/NIH cells stimulated with PDGF

Target description

74 kDa

Physical form

Format: Purified
Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH 7.4.
Protein G Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Expressed in most tissue, lysates from 3T3/NIH cells stimulated with PDGF

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Development of a highly reproducible three-dimensional organotypic model of the oral mucosa.
Anna Dongari-Bagtzoglou, Helena Kashleva
Nature Protocols null
Culture of Drosophila primary cells dissociated from gastrula embryos and their use in RNAi screening.
Bai J, Sepp KJ, Perrimon N
Nature Protocols null
Raf-1 serine 338 phosphorylation plays a key role in adhesion-dependent activation of extracellular signal-regulated kinase by epidermal growth factor.
Edin, ML; Juliano, RL
Molecular and cellular biology null
Regulation of mTORC1 signaling by pH.
Balgi, AD; Diering, GH; Donohue, E; Lam, KK; Fonseca, BD; Zimmerman, C; Numata, M; Roberge, M
Testing null
Antonia K Roseweir et al.
Journal of the National Cancer Institute, 110(6), 616-627 (2018-06-20)
Aromatase inhibitors improve disease-free survival compared with tamoxifen in postmenopausal women with hormone receptor-positive breast cancer. The Tamoxifen and Exemestane Adjuvant Multinational (TEAM) trial compared exemestane monotherapy with sequential therapy of tamoxifen followed by exemestane. The trial failed to show
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