700053P

25-hydroxycholesterol-d6

Avanti Research^™ - A Croda Brand

• Synonym(s): 26,26,26,27,27,27-hexadeuterocholest-5-ene-3β,25-diol; 25-hydroxycholest-5-en-3-ol(d6)
• Empirical Formula (Hill Notation): C₂₇H₄₀D₆O₂
• CAS Number: 88247-69-2
• Molecular Weight: 408.69
• MDL number: MFCD00201435
• UNSPSC Code: 41141804
• NACRES: NA.25

Properties

• description: cholest-5-ene-3β,25-diol-d6
• Assay: >99% (TLC)
• form: powder
• packaging: pkg of 1 × 1 mg (700053P-1mg)
• manufacturer/tradename: Avanti Research^™ - A Croda Brand
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

25-hydroxycholesterol (25HC) is synthesized from cholesterol in the presence of oxygen and enzyme cholesterol 25-hydroxylase and the conversion is dependent on nicotinamide adenine dinucleotide phosphate (NADPH) during toll-like receptor activation. 25HC is catabolized to 7α-hydroxylated sterol. 25-hydroxycholesterol-d6 is the deuterated form of 25-hydroxycholesterol.

Application

25-hydroxycholesterol-d6 has been used as an internal standard:

• in the quantification of 25-OHC in jejunum samples by liquid chromatography with tandem mass spectrometry (LC-MS-MS)
• in plasma samples by liquid chromatography-mass spectrometry analysis
• for calibration curve generation for plasma oxysterol quantification

Biochem/physiol Actions

25-hydroxycholesterol is a liver X receptor ligand. This bioactive lipid regulates immune response and macrophages production from monocytes. 25-HC serves as an antiviral effector and may have therapeutic potential. It reduces cholesterol biosynthesis by inactivating sterol regulatory element binding protein and inhibits HMG-CoA reductase (HMGCR). 25-HC may play a protective role in myocardial ischemia-reperfusion injury by inhibiting apoptosis and regulating mitogen-activated protein kinase (MAPK) pathway.

Packaging

5 mL Amber Glass Screw Cap Vial (700053P-1mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• Flash Point(F): No data available
• Flash Point(C): No data available