ZRB1628
Anti-S100-B Antibody, clone 8F20 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
clone
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 10.71 kDa
observed mol wt ~10 kDa
species reactivity
rat, mouse, human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 8F20 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects S100-B.
Immunogen
His-tagged full-length recombinant human S100-B protein.
Application
Quality Control Testing
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected S100-B in Rat Brain tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected S100-B in mouse spinal cord, human brain, and A375 lysates.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected S100-B in human cerebellum tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected S100-B in A375 cells.Flow Cytometry Analysis: 1 μg from a representative lot detected S100-B in one million C6 cells.Affinity Binding Assay: A representative lot of this antibody bound S-100 B protein with a KD of 3.2 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected S100-B in Rat Brain tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected S100-B in mouse spinal cord, human brain, and A375 lysates.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected S100-B in human cerebellum tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected S100-B in A375 cells.Flow Cytometry Analysis: 1 μg from a representative lot detected S100-B in one million C6 cells.Affinity Binding Assay: A representative lot of this antibody bound S-100 B protein with a KD of 3.2 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-S100-B, clone 8F20 ZooMAb, Cat. No. ZRB1628, is a recombinant Rabbit monoclonal antibody that specifically targets S100-B and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Target description
Protein S100-B (UniProt: P04271; also known as S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B) is encoded by the S100B gene (Gene ID: 6285) in human. S100-B is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. It exists in both heterodimeric and homodimeric forms. Existence of dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain has been reported and dimerization seems to be essential for its biological activities. S100-B has been suggested to play a part in a variety of cellular processes, primarily via binding to key synaptic proteins and inhibiting their phosphorylation. The extracellular form of S100-B, secreted by astrocytes, is physiologically involved in the development and maintenance of CNS homeostasis. S100-B effects are closely dependent on its cellular levels. At nanomolar concentrations it is shown to enhance survival of neurons during development and stimulate neurite outgrowth in cerebral cortex neurons. It also reduces the loss of mitochondrial function and cell death. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Donato, R. (2001). Int. J. Biochem. Cell Biol 33(7); 637-668; Rezaei, O., et al. (2017). Iran J. Neurol. 16(2); 83-89).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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