T0256

Trypsin inhibitor from bovine pancreas

Type I-P, essentially salt-free, lyophilized powder

• Synonym(s): BPTI
• CAS Number: 9087-70-1
• EC Number: 232-906-9
• MDL number: MFCD00082102
• UNSPSC Code: 12352202

Properties

• biological source: bovine pancreas
• type: Type I-P
• form: essentially salt-free, lyophilized powder
• mol wt: ~6500 Da (single-chain 58-amino acid peptide)
• purified by: crystallization
• technique(s): inhibition assay: suitable
• solubility: water: 10 g/L
• storage temp.: −20°C

Description

Biochem/physiol Actions

A 58-residue protein whose binding to various serine proteases has been extensively studied. It is highly homologous to a family of mamba snake dendrotoxin proteins that inhibit various K⁺ channels. It also binds to an intracellular site associated with the large conductance Ca²⁺-activated K⁺ channel..

Unit Definition

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

Preparation Note

Prepared by method of Kunitz and Northrup, J. Gen. Physiol., 19, 991 (1936).

Analysis Note

One mg of trypsin inhibitor will inhibit greater than 1.5 mg of trypsin with activity of approximately 10,000 BAEE units per mg protein.

Protein determined by biuret.

Other Notes

View more information on Trypsin Inhibitor.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves