SAE0102

Antithrombin III human

recombinant,carrier-free,expressed in HEK 293 cells

• Synonym(s): AT-III, AT3, AT3D, ATIII, ATIII-R2, Factor Xa inhibitor, Heparin cofactor, SERPINC1, Serpin family C member1, Serpin-C1, THPH7
• UNSPSC Code: 12352202
• NACRES: NA.41

Properties

• recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• quality: IC₅₀ ≤ 5 nM antithrombin III
• impurities: ≤1.0 EU/μg (LAL test)
• UniProt accession no.: P01008
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Measured by the ability of antithrombin III to inhibit the activity of thrombin in the presence of heparin.

Recombinant human antithrombin III (AT-III) is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 49 kDa (amino acids 33-464). This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.

Application

This recombinant human AT-III product can be used to study the mode of action of the enzyme, as well as its potential inhibitors. It may also be used as a standard, e.g. in antithrombin activity assay, and/or in in vivo studies.

Biochem/physiol Actions

Antithrombin III (AT-III), or Serpin C1 is a serine protease inhibitor glycoprotein, is a member of the Serpin superfamily. Antithrombin III inactivates several components in the coagulation system, IIa,IXa and mainly thrombin and factor Xa. The inhibitory activity of Antithrombin III is potentiated and increased by the polysaccharide heparin. In addition to its abolishment of coagulation, Antithrombin III was also found to suppress proinflammatory mediators, in part by its anticoagulation action. Congenital antithrombin deficiency results in loss of Antithrombin III activity, and leads to thrombosis. Thus, human Antithrombin III enzyme may be prescribed for the treatment and prevention of thromboembolism, as well as for the prevention of perioperative and peripartum thromboembolism.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable