SAB5900206

Alpaca Anti-EGFR, recombinant single domain Antibody

recombinant Alpaca (VHH) Nano antibody

• UNSPSC Code: 12352203

Properties

• biological source: alpaca
• Quality Level: 100
• recombinant: expressed in (E. coli)
• conjugate: unconjugated
• antibody form: (Affinity purified VHH antibody)
• clone: EB61, monoclonal
• Assay: >95% (SDS-PAGE)
• form: lyophilized solid
• mol wt: 14.4 kDa; ~15-18 kDa (SDS PAGE under reducing condition)
• purified by: affinity chromatography (purified via Ni-charged resin)
• species reactivity: human
• concentration: 1 mg/mL
• technique(s): ELISA: 1:1000-1:25000; immunofluorescence: 1:200-1:600; immunohistochemistry: 1:200-1:600
• color: colorless
• pH: ( in PBS, pH 7.4)
• isotype: VHH
• Protein ID accession no.: AAI18666.1
• UniProt accession no.: P00533
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... EGFR(1956)

Description

General description

EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. Growth factor receptors are involved not only in promoting the proliferation of normal cells but also in the aberrant growth of many types of human tumors. Over-expression of the EGFR gene occurs in carcinomas with and without gene amplification. EGFR and erbB-2 are particularly important in breast cancer because increased production or activation has been associated with poor prognosis. EGFR belongs to a family of growth factor receptors, which also includes ErbB-2/HER-2/neu, ErbB-3/HER-3/neu and ErbB-4/HER-4neu. EGFR can heterodimerize with each of the members of this family.

Specificity

Human Epidermal Growth Factor Receptor 1 (HER1/EGFR)

Immunogen

Recombinant human EGFR (Leu25-Ser645) protein

Application

ELISA, IHC,IF

Packaging

50 µg per vial / 100 µg per vial

Physical form

Purified VHH protein in buffer containing 25 mM TAPS pH8.5, 500 mM NaCl, 5 mM EDTA

Reconstitution

Reconstitute the lyophilized protein with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Storage and Stability

Stable for 1 years at 4°C from date of receipt.

Other Notes

Storage at 4°C; Do not freeze.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable