SAB4200767

Anti-Cathepsin D antibody, Mouse monoclonal

clone CTD-19, purified from hybridoma cell culture

• Synonym(s): Anti-CTSD, cleaved into the following 2 chains: Cathepsin D light chain and Cathepsin D heavy chain
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 300
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: CTD-19, monoclonal
• form: buffered aqueous solution
• species reactivity: human, rabbit
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 2-4 μg/mL using human breast cancer MCF7 cell line; immunofluorescence: 5-10 μg/mL using HeLa cells; immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human liver sections
• isotype: IgG2a
• UniProt accession no.: P07339
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CTSD(1509)

Description

General description

Anti-Cathepsin D antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the CTD-19 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with cathepsin D purified from human liver. Cathepsin D (CatD or CTSD) is synthesized in the rough endoplasmic reticulum as pre-proprotein. After removal of signal peptide, the pro-CatD is targeted to endosomes to form an active, ~48 kDa, single-chain intermediate then to the lysosomes to form the fully active mature protease, composed of a ~30 kDa heavy chain and a ~14 kDa light chain.

Immunogen

Cathepsin D Purified from human liver

Application

Anti-Cathepsin D antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunohistochemistry
• immunofluorescence

Biochem/physiol Actions

CatD plays numerous physiological functions in the cells including metabolic degradation of intracellular proteins and the activation of enzymatic precursors. In the central nervous system, CatD is particularly important for the control of neuronal homeostasis, cell migration and interneuron communication. CatD-mediated proteolysis mediates the degradation of unfolded/oxidized protein aggregates in lysosome. The level of CatD synthesized by the cells is increased in response to mitogenic signals from estrogen, epidermal growth factor (EGF), fibroblast growth factor (FGF), and insulin like growth factor-I (IGF- I). The ability of tumor cells to invade the extracellular matrix has been attributed to cathepsins released by tumor cells or associated with its plasma membrane.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable