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feature
DNA free (removal without the use of enzyme)
storage condition
room temperature
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
technique(s)
RT-PCR: suitable
greener alternative category
shipped in
ambient
storage temp.
room temp
General description
Sigma′s GenElute™ Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, plasma, serum, bacteria, yeast, fungi, plants and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
- Isolate true total RNA including siRNA and microRNA
- Rapidly remove contaminating genomic DNA without the use of enzymes
- No phenol or chloroform extractions
- Purify high-quality RNA in 20 minutes
- Extract RNA from as little as a single cell
- Isolate from a wide variety of specimens
Application
GenElute™ Total RNA Purification Kit has been used to:
- Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
- RT-PCR for both large mRNA and small RNA including miRNA
- Expression profiling
- Next Generation Sequencing for RNA and miRNA
- miRNA from plasma for discovery
- microRNA cloning and amplification
- PCR-based virus detection
- PCR-based viable bacteria detection
- Northern blotting
- RNase protection
- Primer extension
- miRNA array
- RNA-Seq
- Nanostring
- Fluidigm
- Droplet/Digital PCR
- Isolate total RNA of rat kidney tissues
GenElute™ Total RNA Purification Kit is used for the extraction of total RNA:
- From whole blood samples in the detection of Hepatitis C Virus using a reverse transcriptase-polymerase spiral reaction (RT-PSR) system.
- From an overnight culture of bacterial strains in TSB medium in determining the effects of curcumin and silymarin on virulence and carbapenemase genes among multidrug-resistant Escherichia coli clinical isolates.
Features and Benefits
- No phenol: chloroform extractions - Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform
- Isolate total RNA from very small samples - Total RNA has been isolated and detected from as little as a single animal cell
- Extremely efficient isolation of low abundance microRNA - GenElute′s Total RNA Purification Kit has been shown to be extremely efficient at recovering low abundance microRNA from plasma samples
- Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
- Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes
- Isolate total RNA from a broad input source - Total RNA has been isolated from cultured animal cells, small tissue samples, LCM samples, bacteria, yeast, fungi, plants, viruses and various bodily fluid including blood, plasma, serum, saliva, nasal or throat swabs
- No need for carrier RNA - Isolate all sizes of RNA without the use of carrier RNA
- Multiple kit sizes available - This kit is available in both 50 prep and 100 prep sizes
Unit Definition
Preps
Other Notes
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
8A - Combustible, corrosive hazardous materials
Certificates of Analysis (COA)
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Protocols
Instructions for purifying viral RNA using GenElute™ RNA purification spin prep kits.
Instructions for purifying viral RNA using GenElute™ RNA purification spin prep kits.
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