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Key Documents

RAB0518

Sigma-Aldrich

Cell-Based ELISA for detecting Phosphotyrosine

in cultured cell line, 96 assays

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

usage

96 assays

species reactivity

human, mouse, rat

technique(s)

ELISA: suitable
capture ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

General description

The Cell-Based Phosphotyrosine ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of Phosphotyrosin and screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.

Immunogen

KLH-conjugated phosphotyramine

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABBLOCK5x Blocking Solution (Item F)SDS

  • RABCTYRODFixing Solution for Phosphotyrosine Assay (Item D)SDS

  • RABPLATE1Uncoated MicroplateSDS

  • RABQUENCHQuenching Solution for Cell-based ELISA Assay (Item E)SDS

  • RABSTOP1Stop SolutionSDS

  • RABTMB1TMB Substrate Reagent (Item J)SDS

  • RABTYROEHRP-Conjugated Phosphotyrosine-specific Antibody Concentrate (Item E)SDS

  • RABWASH120X Wash Buffer Concentrate ASDS

  • RABWASH220X Wash Buffer Concentrate BSDS

See All (9)

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Skin Corr. 1B - STOT SE 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3


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