P1995

Anti-Podoplanin (HG-19) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• MDL number: MFCD05665247
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~38 kDa
• species reactivity: rat
• technique(s): indirect immunofluorescence: 10-20 μg/mL using rat kidney frozen sections; western blot (chemiluminescent): 2.5-5 μg/mL using whole extract of rat kidney glomeruli and rat lung
• UniProt accession no.: Q64294
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Pdpn(54320)

Description

General description

Podoplanin is part of the type-1 transmembrane sialomucin-like glycoprotein family. The gene encoding this 43kDa protein is localized on rat chromosome 5.

Podoplanin is present on the surface of podocytes in kidney glomeruli and the parietal cells of the glomerular Bowman′s capsule. It is also named E11 antigen and has been described to be present in normal, inflammatory, and neoplastic lymphatic capillary endothelium. Podoplanin is expressed selectively by dermal microvascular lymphatic endothelial cells but not by blood vascular endothelial cells. It is composed of heavily glycosylated extracellular domain and a short intracytoplasmic tail.

Immunogen

synthetic peptide of rat podoplanin (amino acids 111-129) with N-terminal cysteine, conjugated to KLH. The sequence in mouse differs by one residue.

Application

Anti-Podoplanin (HG-19) antibody produced in rabbit has been used in immunostaining, immunoblotting and immunofluorescence staining.

Biochem/physiol Actions

Podoplanin expression is regulated by the lymphatic-specific homeobox gene Prox-1. Podoplanin appears to play an important role in the maintenance of podocyte foot processes, and hence, glomerular permeability. It has a critical regulatory role in the formation of the lymphatic vasculature. Podoplanin does not seem to be upregulated in tumor angiogenesis. It is detectable in human angiosarcomas, lymphangiomas, and in Kaposi′s sarcomas.

Podoplanin is a marker of lymphatic endothelial cells. It may have a role in tumor cell invasion and migration.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable