N6655

Anti-Nerve Growth Factor 2.5S antibody produced in rabbit

fractionated antiserum, buffered aqueous solution

• Synonym(s): Anti-NGF-2.5S
• EC Number: 210-123-3
• MDL number: MFCD00162708
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: fractionated antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: mouse, human
• technique(s): Ouchterlony double diffusion: suitable; western blot: 1:2,000
• UniProt accession no.: P01138
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NGF(4803) ; mouse ... Ngf(18049)

Description

General description

Nerve growth factor (NGF) belongs to the family of neurotrophic factors. It is located at 1p13.2 on the human chromosome. There are two molecular forms of NGF, 7S NGF and 2.5S NGF, that have identical neurotrophic effect on neurons.

Specificity

May be used in monitoring for NGF (2.5S, β and 7S).

Immunogen

NGF-2.5S from male mouse submaxillary glands.

Application

Anti-Nerve Growth Factor 2.5S antibody may be used for immunoblotting and dot blot at a minimum antibody working dilution of 1:2000. Antibody dilution of 1:1000 was used for immunoblotting using extracts of nucleus raphe magus tissue of rats. The antibody was used for immunofluorescence of mouse astrocytes and for neutralization assays.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Mutations in this gene leads to hereditary sensory and autonomic neuropathies class five (HSAN-V).

Physical form

Solution in phosphate buffered saline.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable