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MAK006

Sigma-Aldrich

Urea Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

General description

The new Urea Assay Kit, MAK471, is now available! Urea is the major end product of nitrogen metabolism in most animals and is produced in a series of reactions in the liver called the urea cycle. In the urea cycle, ammonia is converted to urea, which is carried by blood to the kidneys for elimination from the body. High levels of urea in the blood may indicate renal failure. Urea levels may also be elevated in response to treatment with certain drugs such as corticosteroids or in response to decreased kidney filtration due to dehydration or congestive heart failure. Decreased blood urea levels can occur in response to liver disease or malnutrition.

Application

Urea assay kit has been used to measure the concentration of urea.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of urea concentration in a variety of samples such as serum, plasma, and urine, etc.

Principle

In this assay, Urea concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm) product, proportional to the Urea present.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H317,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Markers of renal function tests.
Gowda S, et al.
North American Journal of Medical Sciences, 2(4), 170-170 (2010)
Urea and ammonia metabolism and the control of renal nitrogen excretion.
Weiner I D, et al.
Journal of the American Society of Nephrology, 10(8), 1444-1458 (2015)
Protein tyrosine phosphatase 1B deficiency in podocytes mitigates hyperglycemia-induced renal injury.
Ito Y, et al.
Metabolism, Clinical and Experimental, 76, 56-69 (2017)
Ammonia metabolism and the urea cycle: function and clinical implications.
Dimski D S.
Journal of Veterinary Internal Medicine, 8(2), 73-78 (1994)
Inhibition of the RhoGTPase Cdc42 by ML141 enhances hepatocyte differentiation from human adipose-derived mesenchymal stem cells via the Wnt5a/PI3K/miR-122 pathway: impact of the age of the donor.
Chaker D, et al.
Stem Cell Research & Therapy, 9(1), 167-167 (2018)
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Protocols

Enzymatic Method for Determining Urea (Phenylalanine Assay)

Assay protocol for the colorimetric detection of urea in biological samples using the urea assay kit.

Enzymatic Hydroxyproline Assay Protocol

Assay protocol for the colorimetric detection of Hydroxyproline in biological samples using the Hydroxypoline assay kit.

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