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M9288

Sigma-Aldrich

Minimum Essential Medium Eagle

With Hanks′ salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Minimum Essential Medium Eagle, 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
HEPES: no
sodium pyruvate: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Minimum Essential Medium (MEM), one of the most used synthetic cell culture media, is developed by Harry Eagle. It is used to cultivate various types of cells grown in monolayers.

Application

Minimum Essential Medium Eagle has been used as a transport media to maintain pig liver cells.

Reconstitution

Supplement with 0.292 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.

also commonly purchased with this product

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Description
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supplement

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Description
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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Simona Giunta et al.
The Journal of cell biology, 190(2), 197-207 (2010-07-28)
The signaling cascade initiated in response to DNA double-strand breaks (DSBs) has been extensively investigated in interphase cells. Here, we show that mitotic cells treated with DSB-inducing agents activate a "primary" DNA damage response (DDR) comprised of early signaling events
Pasqualino de Antonellis et al.
PloS one, 6(9), e24584-e24584 (2011-09-21)
Through negative regulation of gene expression, microRNAs (miRNAs) can function as oncosuppressors in cancers, and can themselves show altered expression in various tumor types. Here, we have investigated medulloblastoma tumors (MBs), which arise from an early impairment of developmental processes
Carlos Mendoza-Palomares et al.
Eukaryotic cell, 7(4), 684-697 (2008-02-19)
Cysteine proteases have been shown to be essential virulence factors and drug targets in trypanosomatids and an attractive antidisease vaccine candidate for Trypanosoma congolense. Here, we describe an important amplification of genes encoding cathepsin B-like proteases unique to T. congolense.
Hisashi Miura et al.
Nucleic acids research, 37(3), 778-792 (2008-12-17)
Mammalian tissue- and/or time-specific transcription is primarily regulated in a combinatorial fashion through interactions between a specific set of transcriptional regulatory factors (TRFs) and their cognate cis-regulatory elements located in the regulatory regions. In exploring the DNA regions and TRFs
Michelle Griffin et al.
PloS one, 6(8), e23404-e23404 (2011-08-23)
Non-unions pose complications in fracture management that can be treated using electrical stimulation (ES). Bone marrow mesenchymal stem cells (BMMSCs) are essential in fracture healing; however, the effect of different clinical ES waveforms on BMMSCs cellular activities remains unknown. We
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Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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