I3909
Ionomycin calcium salt
Ready Made Solution, from Streptomyces conglobatus, 1 mM in DMSO
Synonym(s):
Calcium Ionophore Ionomycin
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About This Item
Empirical Formula (Hill Notation):
C41H70CaO9
Molecular Weight:
747.07
EC Number:
MDL number:
UNSPSC Code:
51102829
PubChem Substance ID:
NACRES:
NA.85
biological source
Streptomyces conglobatus
Quality Level
Assay
≥98% (HPLC)
shelf life
2 yr
storage condition
(Tightly closed. )
concentration
1 mM in DMSO
color
colorless
mp
18.4 °C
solubility
DMSO: 1 mM
antibiotic activity spectrum
Gram-positive bacteria
Mode of action
cell membrane | interferes
shipped in
wet ice
storage temp.
−20°C
SMILES string
C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)C\C=C\[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O
InChI
1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1
InChI key
WKRWUYKLUMMAKG-WYGBAUISSA-L
General description
Ionomycin is a calcium ionophore commonly derived from produced by the bacterium Streptomyces conglobatus, which means that it helps to transport calcium ions across cell membranes. It is a powerful tool for studying calcium signaling and its role in various biological processes, including cell activation, secretion, proliferation, and apoptosis.
Ionomycin is commonly used in cell biology experiments to induce a rapid and controlled increase in intracellular calcium levels. This allows to study the downstream effects of calcium signaling pathways, such as the activation of enzymes, the release of hormones, and the changes in gene expression.Ionomycin is also used to investigate the role of calcium in specific cellular processes, such as cell death, immune cell activation, and muscle contraction. For example, ionomycin can be used to induce apoptosis in cancer cells, study the activation of T cells, and investigate the role of calcium in muscle contraction.
Ionomycin is commonly used in cell biology experiments to induce a rapid and controlled increase in intracellular calcium levels. This allows to study the downstream effects of calcium signaling pathways, such as the activation of enzymes, the release of hormones, and the changes in gene expression.Ionomycin is also used to investigate the role of calcium in specific cellular processes, such as cell death, immune cell activation, and muscle contraction. For example, ionomycin can be used to induce apoptosis in cancer cells, study the activation of T cells, and investigate the role of calcium in muscle contraction.
Application
Ionomycin finds application in:
- Study of calcium signaling and its role in various biological processes
- Investigation of intracellular calcium levels, calcium-dependent enzymes, and cellular responses triggered by calcium influx
- Induction of a rapid and controlled increase in intracellular calcium levels in cell biology experiments
- Elucidation of specific cellular mechanisms and signaling pathways in combination with other substances, such as fluorescent calcium indicators or protein kinase activators
- to induce central demyelination
- inhibit adrenal bovine TREK-1 channels
- to regulate cell division of mature human B cells
Biochem/physiol Actions
Ionomycin is an ionophoric antibiotic that binds Pb2+ and Ca2+, and serves as an effective mobile carrier of both cations.
Mode of Action: Facilitates the transfer of calcium ions (Ca2+) across biological membranes
Antimicrobial Spectrum: Active against Gram-Positive bacteria
Mode of Action: Facilitates the transfer of calcium ions (Ca2+) across biological membranes
Antimicrobial Spectrum: Active against Gram-Positive bacteria
Ionomycin is an ionophoric antibiotic which binds Pb2+ and Ca2+, and serves as an effective mobile carrier of both cations. Ionomycin is more effective than A23187 as a mobile ion carrier for Ca2+. It is non-fluorescent. It is used to study Ca2+ transport across biological membranes and induces apoptotic degeneration of embryonic cortical neurons.
Features and Benefits
- Ready available solution reduce the need for preparation time
- Efficient choice for studying calcium signaling and its role in various biological processes
- Allows for rapid and controlled manipulation of intracellular calcium levels
- Versatile and widely used in cell biology research
Other Notes
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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K J Smith et al.
Brain : a journal of neurology, 117 ( Pt 6), 1351-1356 (1994-12-01)
The effects of injecting the calcium-selective ionophore, ionomycin, into myelinated tracts in the dorsal columns of adult rat spinal cords were examined electron microscopically. In vivo, ionomycin induced a primary vesicular demyelination, together with a variable degree of axonal degeneration
K M Kim et al.
Journal of immunology (Baltimore, Md. : 1950), 148(6), 1797-1803 (1992-03-15)
The growth of a human B lymphoma cell line B104, an experimental model for mature B cells, was inhibited by ionomycin but not 12-O-tetradecanoylphorbol-13-acetate (TPA). Ionomycin inhibited B104 cells from entering into the M phase of the cell cycle without
John J Enyeart et al.
American journal of physiology. Cell physiology, 301(3), C619-C629 (2011-05-27)
Bovine adrenocortical cells express bTREK-1 K(+) (bovine KCNK2) channels that are inhibited by ANG II through a Gq-coupled receptor by separate Ca(2+) and ATP hydrolysis-dependent signaling pathways. Whole cell and single patch clamp recording from adrenal zona fasciculata (AZF) cells
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