G5768
Gamborg′s B-5 Basal Salt Mixture
fine powder, suitable for plant cell culture
Synonym(s):
B-5 Salt Mixture, Basal Salt Mixture, Gamborg B-5 Medium
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.72
Recommended Products
form
fine powder
Quality Level
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
General description
Gamborg′ B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis.
Application
Gamborg′s B-5 Basal Salt Mixture has been used:
- as a constituent during the preparation of TEX buffer
- as a media to maintain potato suspension and callus cultures
- as a media in the primary-node transformation method
Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Formula variant
With the concentrations of macro- and micronutrients as described by Gamborg, et al. (1968).
Media Formulation
Media Formulation
Quantity
Formulated to contain 3.1 grams of powder per liter of medium.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Ox. Sol. 3
Storage Class Code
5.1B - Oxidizing hazardous materials
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
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