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Key Documents

F7524

Sigma-Aldrich

Fetal Bovine Serum

non-USA origin, sterile-filtered, suitable for cell culture

Synonym(s):

Fetal Calf sera, Fetal Calf serum, FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
eCl@ss:
32160409
NACRES:
NA.71

biological source

bovine fetus

Quality Level

400

sterility

sterile-filtered

product line

FBS Classic

composition

hemoglobin, ≤25 mg/dL

origin

non-USA origin

availability

not available in Canada, USA

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

Fetal Bovine Serum has been used as a supplement in cell culture media such as Roswell Park Memorial Institute medium (RPMI-1640) and Dulbecco′s Modified Eagles Media (DMEM)to support the growth of a variety of cell lines.

Biochem/physiol Actions

Fetal bovine serum (FBS) serves as a commonly utilized cell culture media supplement to support in vitro and ex vivo cell, tissue, and organ cultures. FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth, and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems. It provides many non-defined growth-promoting and survival enhancing factors to cells in culture. Derived from clotted blood, it is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. FBS is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Features and Benefits

F7524 is the same great product as Biochrom GmbH. product S0115.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sigma-Aldrich

Sigma-Aldrich

N4637

Newborn Calf Serum

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Choosing the optimal cell culture media for vaccine production

Cultured cells are excellent hosts for the propagation of many types of viruses. The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS).

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