F5512

Anti-Human IgG (Fab specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Goat Anti-Human IgG (Fab specific)−Fluorescein isothiocyanate
• MDL number: MFCD00162433
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: goat
• conjugate: FITC conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• storage condition: protect from light
• technique(s): immunofluorescence: 1:16-1:32 using Hep2 cells
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

The product binds all human Igs and provides reduced background with mouse or rat samples.

Application

Anti-Human IgG (Fab specific)-FITC antibody has been used:

• in fluorescent staining
• in dual color fluorescent labeling
• in fluorescence-activated cell sorting (FACS) analysis

Anti-Human IgG (Fab specific)-FITC antibody produced in goat may be used at a working antibody dilution of 1:16 - 1:32 for anti-nuclear antibody (A.N.A.) assay on acetone-fixed human Hep2 cells. This antibody was used in FACS of HNE2 and KNE2/LMP1 cells. It was also used to perform TARDIS assay for detection of topoisomerases I and II that are covalently bound to DNA in K562 leukemia cells.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti- Human IgG (Fab specific) antibody allows for the visualization of protein by fluorescent microscopy.

Other Notes

No cross-reaction with mouse and rat IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves