E1157

Anti-Epidermal Growth Factor Receptor/Erb B1 antibody produced in goat

affinity isolated antibody, lyophilized powder

• Synonym(s): Anti-EGFR, Anti-ERBB, Anti-HER1, Anti-PIG61
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: goat
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): capture ELISA: 0.4 μg/mL; flow cytometry: 25 μg/mL; immunoprecipitation (IP): 1 μg/mL; western blot: 1-2 μg/mL
• UniProt accession no.: P00533
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EGFR(1956)

Description

Immunogen

purified, NSO cell-derived recombinant human epidermal growth factor receptor extracellular domain.

Application

Anti-Epidermal Growth Factor Receptor/Erb B1 antibody produced in goat was used for immunoblotting to analyze stable expression of anti-EGFR shRNA in MCF10A breast epithelial cell line.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves