DUO94104

Duolink^® flowPLA Mouse/Rabbit Starter Kit - Far Red

Duolink^® PLA kit for Flow Cytometry with Far Red Detection and Mouse/Rabbit probes

• Synonym(s): in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit

Properties

• Quality Level: 200
• technique(s): flow cytometry: suitable; immunofluorescence: suitable; proximity ligation assay: suitable
• fluorescence: λ_ex 644 nm; λ_em 669 nm
• suitability: suitable for fluorescence
• storage temp.: −20°C

Description

Specificity

Far Red Fluorescence Detection Reagents
Use appropriate laser for λ_ex 644 nm excitation
Use appropriate filter for λ_em 669 nm emission.

Application Note

Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Flow validated antibodies are recommended.

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Application

Based on proximity ligation assay (PLA), the Duolink^® PLA technology allows for endogenous detection of protein interactions, post-translational modifications, and protein expression levels at the single molecule level in fixed cells.

Duolink^® flowPLA Starter Kits will enable sensitive detection of proteins, protein-protein interactions, and protein modifications within cell populations by flow cytometry. To perform a Duolink^® flowPLA experiment, you will need fixed, suspended cells, two primary antibodies that specifically recognize your proteins of interest and the Duolink^® flowPLA starter Kit. The flowPLA Starter Kits are available with 3 different fluorophores: Violet, Green, and FarRed. The flowPLA starter Kits contain all the necessary reagents reagents for 100 Duolink^® PLA reactions, including a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS) and all the reagents to perform the amplification and detection of bound PLA probes by flow cytometry. Analysis is carried out using standard flow cytometry assay equipment. User must provide a fixed cell suspension, (rabbit and mouse-raised) primary antibodies, and corresponding PLA Probes.

Follow the Duolink^® PLA Flow Cytometry Protocol to use this product.

Visit our Duolink^® PLA Flow Cytometry page on how to run a Duolink^® flow experiment, applications, troubleshooting, and more.

Features and Benefits

• Analyze protein protein interactions with flow cytometry readout
• Analyze cell populations with Proximity Ligation Assay
• Increased sensitivity due to rolling circle amplification for low abundant targets
• No overexpression or genetic manipulation required
• Relative quantification possible
• Works with any flow cytometer instrumentation
• Easy to follow flexible protocol
• Publication-ready results

Components

Duolink^® flowPLA Detection Kit – Far Red DUO94004-40TST
Duolink^® In Situ PLA^® Probe Anti-Mouse MINUS DUO92004-40TST
Duolink^® In Situ PLA^® Probe Anti-Rabbit PLUS DUO92002-40TST
Duolink^® In Situ Wash Buffer, Brightfield (DUO82047-4L)

See datasheet for more information.

Principle

Duolink^® flow PLA Mouse/Rabbit Starter Kit - Far Red has been used in the proximity ligase assay (PLA) to detect co-localization of human immune deficiency virus (HIV) protein, negative factor (Nef) with serine incorporator (SERINC3(iHA)) in human T-lymphoblastoid CEM A*02 cells.

Legal Information

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany

PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Plexiglas is a registered trademark of Arkema

Safety Information

• Pictograms: GHS07,GHS09
• Signal Word: Warning
• Hazard Statements: H317 - H411
• Precautionary Statements: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Hazard Classifications: Aquatic Chronic 2 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3