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DNASE10

Sigma-Aldrich

On-Column DNase I Digestion Set

sufficient for 10 preparations

Synonym(s):

Deoxyribonuclease I Set

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.52

grade

for molecular biology

form

liquid

usage

sufficient for 10 preparations

technique(s)

RNA purification: suitable

storage temp.

−20°C

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General description

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit and the Spectrum Plant Total RNA Kit. It can also be used with many other commercially available RNA purification kits.
The vast majority of DNA is eliminated from total RNA purifications by the procedures provided in the standard protocols of the Sigma Kits named below. However, for very sensitive applications, such as quantitative RT-PCR, even trace amounts of DNA can result in false positive detection of cDNA-derived amplicons.

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit, the GenElute Bacterial Total RNA Purification Kit, the GenElute Yeast Total RNA Purification Kit and the Spectrum™ Plant Total RNA Kit. However, the digestion protocol is compatible with many other commercially available RNA purification kits.

The term ′On-Column′ indicates that digestion is accomplished in-process for true convenience. Each of Sigma′s total RNA purification kits utilize a binding column for purification. DNase I digestion is accomplished in 15 minutes while total RNA is bound to the column substrate. Several wash steps and elution of the total RNA immediately follow. To review the complete protocol, see the Technical Bulletin for any of the Kits named above.

Application

On-Column DNase I Digestion Set is suitable for removing gDNA from total RNA preparations. It may not be suitable for RT-PCR applications as a small amount of residual DNA may remain. On-Column DNase I Digestion Set was used to purify RNA preparations isolated from Vitis vinifera cultivars.

Features and Benefits

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit, the GenElute Bacterial Total RNA Purification Kit, the GenElute Yeast Total RNA Purification Kit and the Spectrum™ Plant Total RNA Kit. However, the digestion protocol is compatible with many other commercially available RNA purification kits.

Principle

The on-column digestion is accomplished in-process for convenience. DNase I digestion is accomplished in 15 minutes while total RNA is bound to the column substrate. Several wash steps and elution of the total RNA immediately follow.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC
Spectrum is a trademark of Sigma-Aldrich Co. LLC

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Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Michal Bukowski et al.
Nature communications, 4, 2012-2012 (2013-06-19)
Toxin-antitoxin systems were shown to be involved in plasmid maintenance when they were initially discovered, but other roles have been demonstrated since. Here we identify and characterize a novel toxin-antitoxin system (pemIKSa) located on Staphylococcus aureus plasmid pCH91. The toxin
Carmen Michán et al.
FEMS yeast research, 9(7), 1078-1090 (2009-08-26)
We report the absolute transcription profiles of 24 genes coding for putative thioredoxin (Trx)- and glutathione (GSH)-dependent redox system components, accompanying the Candida albicans batch culture growth, under either yeast or hyphal conditions. All mRNAs investigated (plus the housekeeping ACT1)
Filipa Monteiro et al.
PloS one, 8(9), e72998-e72998 (2013-09-12)
The pivotal role of cultivated grapevine (Vitis vinifera L.) in many countries economy is compromised by its high susceptibility to Plasmopara viticola, the causal agent of downy mildew disease. Recent research has identified a set of genes related to resistance

Protocols

Viral RNA Purification Protocol Using GenElute™ Spin Prep Kits

Instructions for purifying viral RNA using GenElute™ RNA purification spin prep kits.

Viral RNA Purification Protocol Using GenElute™ Spin Prep Kits

Instructions for purifying viral RNA using GenElute™ RNA purification spin prep kits.

Related Content

Spectrum™ Plant Total RNA Kit

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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