All Photos(3)

Key Documents

COO/COA

3,4-Dichloroisocoumarin

Name: 3,4-Dichloroisocoumarin
Brand: SIGMA

serine protease inhibitor

Synonym(s):

3,4-DCI, 3,4-Dichloro-2-benzopyran-1-one

Sign Into View Organizational & Contract Pricing

All Photos(3)

About This Item

Empirical Formula (Hill Notation):

C₉H₄Cl₂O₂

CAS Number:

51050-59-0

Molecular Weight:

215.03

Beilstein:

6802625

MDL number:

MFCD00036960

UNSPSC Code:

12352202

PubChem Substance ID:

24278386

NACRES:

NA.77

Quality Level

200

Assay

≥98% (TLC)

form

powder

solubility

ethyl acetate: 49.00-51.00 mg/mL, clear, colorless to faintly yellow

storage temp.

2-8°C

SMILES string

ClC1=C(Cl)c2ccccc2C(=O)O1

InChI

1S/C9H4Cl2O2/c10-7-5-3-1-2-4-6(5)9(12)13-8(7)11/h1-4H

InChI key

SUGXUUGGLDCZKB-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

Useful in a rapid staining method for identification of macrophages; counts by this method were confirmed by the more complex morphological criteria, by phagocytosis, and by the presence of Fc receptors.

Other Notes

Effective concentration 5-100 μM.

Quantity

Half-life = 20 min at pH 7.5.

Substrates

Serine protease inhibitor. Active towards a wide range of serine proteases including granzymes. Not active toward β-lactamases.

Pictograms

GHS06

Signal Word

Danger

Hazard Statements

H301,H315,H319,H335

Precautionary Statements

P301 + P310 + P330 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 3 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Serine protease inhibitors block invasion of host cells by Toxoplasma gondii.

V Conseil et al.

Antimicrobial agents and chemotherapy, 43(6), 1358-1361 (1999-05-29)

We investigated the effect of protease inhibitors on the asexual development of the protozoan parasite Toxoplasma gondii. Among the inhibitors tested only two irreversible serine protease inhibitors, 3,4-dichloroisocoumarin and 4-(2-aminoethyl)-benzenesulfonyl fluoride, clearly prevented invasion of the host cells by specifically

Release of signal peptide fragments into the cytosol requires cleavage in the transmembrane region by a protease activity that is specifically blocked by a novel cysteine protease inhibitor.

A Weihofen et al.

The Journal of biological chemistry, 275(40), 30951-30956 (2000-08-02)

Signal peptides of secretory and membrane proteins are generated by proteolytic processing of precursor proteins after insertion into the endoplasmic reticulum membrane. Liberated signal peptides can be further processed, and the resulting N-terminal fragments are released toward the cytosol, where

Cardiovascular and inflammatory mechanisms in healthy humans exposed to air pollution in the vicinity of a steel mill.

Premkumari Kumarathasan et al.

Particle and fibre toxicology, 15(1), 34-34 (2018-08-12)

There is a paucity of mechanistic information that is central to the understanding of the adverse health effects of source emission exposures. To identify source emission-related effects, blood and saliva samples from healthy volunteers who spent five days near a

Rhomboid-Like-2 Intramembrane Protease Mediates Metalloprotease-Independent Regulation of Cadherins.

Chiara Battistini et al.

International journal of molecular sciences, 20(23) (2019-12-01)

Cadherins are a major family of cell-cell adhesive receptors, which are implicated in development, tissue homeostasis, and cancer. Here, we show a novel mechanism of post-translational regulation of E-cadherin in cancer cells by an intramembrane protease of the Rhomboid family

Granzyme B Attenuates Bacterial Virulence by Targeting Secreted Factors.

Diego López León et al.

iScience, 23(3), 100932-100932 (2020-03-11)

Pathogenic bacteria secrete virulence factors that interact with the human host to establish infections. The human immune system evolved multiple mechanisms to fight bacterial invaders, including immune proteases that were demonstrated to contribute crucially to antibacterial defense. Here we show

View All Related Papers

Articles

Elastase

Enzyme Explorer Product Application Index for Elastase. Leukocyte elastase is a 29KDa serine endoprotease of the Proteinase S1 Family. It exists as a single 238 amino acid-peptide chain with four disulfide bonds.

Papain

Papain is a cysteine protease of the peptidase C1 family. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.