CWDS-AF488

ColorWheel^® Antibody-Ready Alpha Fluor 488 Dye

for use with ColorWheel^® Dye-ready Antibodies (Required, sold separately)

• UNSPSC Code: 41116015

Properties

• product line: ColorWheel^®
• Quality Level: 200
• form: lyophilized
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• technique(s): flow cytometry: suitable
• fluorescence: λ_ex 499 nm; λ_em 520 nm
• compatibility: for use with ColorWheel^® Dye-ready Antibodies (Required, sold separately)
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Alpha Fluor^™ 488 NHS Ester (Succinimidyl Ester) is a water soluble, bright green fluorescent dye that exhibits an excitation max at 499 nm and an emission max at 520 nm with an extinction coefficient of 73,000 cm-1 M-1. It is suitable for use over a wide range of pH (4 to 10). It can be used with the 488 nm Argon laser and its spectral properties are identical to Alexa Fluor^™ 488. The NHS ester is the most convenient amine-reactive form for conjugating it to proteins and antibodies and can be used for stable signal generation in Flow cytometry. It displays high photostability and brighter fluorescence.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Application

Flow Cytometry: Excitation: 499 nm, Emission: 520 nm

Compatibility

Requires ColorWheel^® Antibody (Sold Separately)

Physical form

CWDS-AF488-25UL Set Components

CWD-AF488-25UL - ColorWheel^® Antibody-Ready Alpha Fluor 488 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

CWD-FINISH-25UL -ColorWheel^® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.

Reconstitution

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3